Data/hora: 01/06/2024 13:21:41

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins	
Autores: 	Solís-Guzmán,María Gloria; Argüello-Astorga,Gerardo; López-Bucio,José; Ruiz-Herrera,León Francisco; López-Meza,Joel; Sánchez-Calderón,Lenin; Carreón-Abud,Yazmín; Martínez-Trujillo,Miguel.	
Data: 	2017-09-01	
Ano: 	2017	
Palavras-chave: 	Spen; Arabidopsis; Vascular bundle; Expression.	
Resumo: 	Abstract Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its promoter region with uidA::gfp reporter genes; the expression was observed in vascular tissues of leaves and roots, as well as in ovules and developing embryos. There was absence of a notable phenotype in knockout and overexpressing lines, suggesting that its function in plants might be specific to certain endogenous or environmental conditions. Our results suggest that the function of Atspen2 diverged from that of fpa due in part to their different transcription expression pattern and divergence of the regulatory SPOC domain.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400643	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/1678-4685-gmb-2016-0223	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.40 n.3 2017	
Fonte: 	info:eu-repo/semantics/openAccess