Data/hora: 01/06/2024 04:03:49

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis	
Autores: 	Chiang,Hsin-I; Berghman,Luc R.; Zhou,Huaijun.	
Data: 	2009-01-01	
Ano: 	2009	
Palavras-chave: 	RNA interference; NF-kBp50; Macrophage; Salmonella; Chicken.	
Resumo: 	The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a 36% inhibition of NF-kB 1 expression, gene expression of both Toll-like receptor (TLR) 4 and interleukin (IL)-6 was consistently and significantly increased at both 1 h and 4 h following SE challenge, whereas the gene expression of MyD88 and IL-1β was increased at 1 h and 4 h, respectively. These findings suggest a likely inhibitory regulation by NF-kB 1, and could lay the foundation for studying the gene network of the innate immune response of SE infection in chickens.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572009000300013	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.32 n.3 2009	
Fonte: 	info:eu-repo/semantics/openAccess