Data/hora: 14/06/2024 07:06:07

Provedor de dados: | 74
País: | Brazil
Título: | A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques
Autores: | Bione,Edgar; Camparoto,Marjori Leiva; Simões,Zilá Luz Paulino.
Data: | 2005-03-01
Ano: | 2005
Palavras-chave: | Coleoptera; Heterochromatin; NORs; FISH.
Resumo: | Meiotic and mitotic chromosomes of Isocopris inhiata and Diabroctis mimas were studied by standard staining procedures, C-banding, silver nitrate staining and FISH using Apis mellifera 28S rDNA as probe. Isocopris inhiata presented a 2n = 18 (8II+ Xy p) karyotype, composed of meta-submetacentric chromosomes with gradual reduction in size. The karyotype of D. mimas was 2n = 20 (9II+ Xy p), composed of meta-submetacentric (pairs 1, 2, 3, 4 and 7) and acrocentric (pairs 5, 6, 8 and 9) chromosomes, with gradual reduction in size. Analysis of constitutive heterochromatin revealed similar C-banding patterns for both species, showing pericentromeric and telomeric bands and diphasic chromosomes. In addition, the X chromosomes of these species were found to be almost completely heterochromatic. The presence of chromocenters was checked in one or more phases of prophase I of these species. All heterochromatin reacted positively for the silver stain. By FISH analysis we were able to locate the rDNA in medium-size autosome pairs in both species and in the X chromosome of D. mimas.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100019
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572005000100019
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.28 n.1 2005
Fonte: | info:eu-repo/semantics/openAccess