Data/hora: 01/06/2024 14:24:08

Provedor de dados: | 74
País: | Brazil
Título: | Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Autores: | Inglis,Peter W.; Ciampi,Ana Y.; Salomão,Antonieta N.; Costa,Tânia da S.A.; Azevedo,Vânia C.R.
Data: | 2014-01-01
Ano: | 2014
Palavras-chave: | Microgravity; Stress response; Germination; Suppression subtractive hybridization; Zebrawood.
Resumo: | Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the microgravity-treated seedlings compared to terrestrially germinated controls. Suppression subtractive hybridization of cDNA and EST analysis were used to detect differential gene expression in the microgravity-treated seedlings in comparison to those initially grown in normal gravity (forward subtraction). Despite their return to, and growth in normal gravity, the subtracted library derived from microgravity-treated seedlings was enriched in known microgravity stress-related ESTs, corresponding to large and small heat shock proteins, 14-3-3-like protein, polyubiquitin, and proteins involved in glutathione metabolism. In contrast, the reverse-subtracted library contained a comparatively greater variety of general metabolism-related ESTs, but was also enriched for peroxidase, possibly indicating the suppression of this protein in the microgravity-treated seedlings. Following continued growth for 30 days, higher concentrations of total chlorophyll were detected in the microgravity-exposed seedlings.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100014
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572014000100014
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.37 n.1 2014
Fonte: | info:eu-repo/semantics/openAccess