Data/hora: 05/06/2024 02:50:32

Provedor de dados: | 74
País: | Brazil
Título: | Heterochromatin analysis in the fish species Liposarcus anisitsi (siluriformes) and Leporinus elongatus (characiformes)
Autores: | Artoni,Roberto Ferreira; Molina,Wagner Franco; Bertollo,Luis Antonio Carlos; Galetti Junior,Pedro Manoel.
Data: | 1999-03-01
Ano: | 1999
Resumo: | The chromosomes of two neotropical freshwater fish species, namely Liposarcus anisitsi (Siluriformes, Loricariidae) and Leporinus elongatus (Characiformes, Anostomidae), were investigated by means of C-banding, Ag-NORs, fluorochrome staining and banding by hot saline solution (HSS) treatment, to reveal patterns of heterochromatin differentiation. The karyotype of L. anisitsi is described for the first time. Staining with the GC-specific fluorescent antibiotic mithramycin (MM) revealed bright signals in some C-banded blocks in both species, suggesting that these MM+ heterochromatin contains GC-rich DNA. Banding by denaturation employing HSS, followed by Giemsa staining, yielded corresponding results documenting the thermal stability of GC-rich DNA part of heterochromatin positive after C-banding. In L. elongatus the Ag-NOR also followed the above banding patterns. However, in L. anisitsi the Ag-NOR was MM+ but negatively stained after C-banding and HSS treatment. L. elongatus also showed C-banded segments that were negative for mithramycin staining and HSS treatment. The results obtained evidence the heterochromatin heterogeneity in these fish species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100009
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47571999000100009
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.22 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess