Data/hora: 09/06/2024 15:33:50

Provedor de dados: | 74
País: | Brazil
Título: | A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
Autores: | Santelli,Roberto V.; Navarro-Cattapan,Luci Deise.
Data: | 2000-03-01
Ano: | 2000
Resumo: | A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572000000100011
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.23 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess