Data/hora: 14/06/2024 19:40:11

Provedor de dados: | 74
País: | Brazil
Título: | Estimating genomic instability mediated by Alu retroelements in breast cancer
Autores: | Fazza,Ana Cristina; Sabino,Flavia Cal; Setta,Nathalia de; Bordin Jr.,Newton Antonio; Silva,Eloiza Helena Tajara da; Carareto,Claudia Marcia Aparecida.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Alu-PCR; Breast cancer; Gene insertions; Gene deletions; Invasive ductal carcinoma; Recombination.
Resumo: | Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009005000018
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess