Data/hora: 01/06/2024 19:01:06

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene	
Autores: 	Ribon,Andréa de O.B.; Ribeiro,João Batista; Gonçalves,Daniel B.; Queiroz,Marisa V. de; Araújo,Elza F. de.	
Data: 	2009-01-01	
Ano: 	2009	
Palavras-chave: 	Penicillium griseoroseum; Polygalacturonase; 5' upstream regulatory sequence; Electrophoretic mobility shift assay.	
Resumo: 	Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100019	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572009005000021	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.32 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess