Data/hora: 01/06/2024 11:36:25

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Sequence and expression pattern of the germ line marker vasa in honey bees and stingless bees	
Autores: 	Tanaka,Érica Donato; Hartfelder,Klaus.	
Data: 	2009-01-01	
Ano: 	2009	
Palavras-chave: 	Apis mellifera; Melipona quadrifasciata; Vasa; Social insect; Oogenesis.	
Resumo: 	Queens and workers of social insects differ in the rates of egg laying. Using genomic information we determined the sequence of vasa, a highly conserved gene specific to the germ line of metazoans, for the honey bee and four stingless bees. The vasa sequence of social bees differed from that of other insects in two motifs. By RT-PCR we confirmed the germ line specificity of Amvasa expression in honey bees. In situ hybridization on ovarioles showed that Amvasa is expressed throughout the germarium, except for the transition zone beneath the terminal filament. A diffuse vasa signal was also seen in terminal filaments suggesting the presence of germ line cells. Oocytes showed elevated levels of Amvasa transcripts in the lower germarium and after follicles became segregated. In previtellogenic follicles, Amvasa transcription was detected in the trophocytes, which appear to supply its mRNA to the growing oocyte. A similar picture was obtained for ovarioles of the stingless bee Melipona quadrifasciata, except that Amvasa expression was higher in the oocytes of previtellogenic follicles. The social bees differ in this respect from Drosophila, the model system for insect oogenesis, suggesting that changes in the sequence and expression pattern of vasa may have occurred during social evolution.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300023	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572009005000043	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.32 n.3 2009	
Fonte: 	info:eu-repo/semantics/openAccess