Data/hora: 05/06/2024 18:04:14

Provedor de dados: | 74
País: | Brazil
Título: | MLL leukemia-associated rearrangements in peripheral blood lymphocytes from healthy individuals
Autores: | Brassesco,María Sol; Montaldi,Ana Paula; Gras,Diana Ester; Queiroz,Rosane Gomes de Paula; Martinez-Rossi,Nilce Maria; Tone,Luiz Gonzaga; Sakamoto-Hojo,Elza Tiemi.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Genomic instability; Lymphocytes; MLL rearrangements.
Resumo: | Chromosomal translocations are characteristic of hematopoietic neoplasias and can lead to unregulated oncogene expression or the fusion of genes to yield novel functions. In recent years, different lymphoma/leukemia-associated rearrangements have been detected in healthy individuals. In this study, we used inverse PCR to screen peripheral lymphocytes from 100 healthy individuals for the presence of MLL (Mixed Lineage Leukemia) translocations. Forty-nine percent of the probands showed MLL rearrangements. Sequence analysis showed that these rearrangements were specific for MLL translocations that corresponded to t(4;11)(q21;q23) (66%) and t(9;11) (20%). However, RT-PCR failed to detect any expression of t(4;11)(q21;q23) in our population. We suggest that 11q23 rearrangements in peripheral lymphocytes from normal individuals may result from exposure to endogenous or exogenous DNA-damaging agents. In practical terms, the high susceptibility of the MLL gene to chemically-induced damage suggests that monitoring the aberrations associated with this gene in peripheral lymphocytes may be a sensitive assay for assessing genomic instability in individuals exposed to genotoxic stress.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200005
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009000200005
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess