Data/hora: 05/06/2024 15:31:45

Provedor de dados: | 74
País: | Brazil
Título: | Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera)
Autores: | Tavares,Mara Garcia; Azeredo-Oliveira,Maria Tercilia Vilela de; Ceron,Carlos Roberto.
Data: | 1998-12-01
Ano: | 1998
Resumo: | We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively inhibited by phenylmethylsulfonyl fluoride (PMSF). Absence of genetic variability could be due to high inbreeding.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47571998000400009
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.21 n.4 1998
Fonte: | info:eu-repo/semantics/openAccess