Data/hora: 10/06/2024 04:57:09

Provedor de dados: | 74
País: | Brazil
Título: | DNA tagging of blast resistant gene(s) in three Brazilian rice cultivars
Autores: | Sandhu,S.S.; Colombo,Carlos; Bastos,Cândido R.; Siqueira,Walter J.
Data: | 2003-12-01
Ano: | 2003
Palavras-chave: | Rice; SCAR; Arbitrary primers; Pyricularia oryzae.
Resumo: | Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572003000400011
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.26 n.4 2003
Fonte: | info:eu-repo/semantics/openAccess