Data/hora: 13/06/2024 16:07:30

Provedor de dados: | 74
País: | Brazil
Título: | DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
Autores: | Lima,Patrícia Santos Pereira; Molffeta,Greice Andreoti; Araujo,Amélia Góes de; Zago,Marco Antônio; Silva Jr.,Wilson Araújo da.
Data: | 2008-01-01
Ano: | 2008
Palavras-chave: | CpG island; DNA methylation; CDKN2B.
Resumo: | The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008000400005
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.3 2008
Fonte: | info:eu-repo/semantics/openAccess