Data/hora: 15/06/2024 23:58:54

Provedor de dados: | 74
País: | Brazil
Título: | Molecular cloning and expression profiling of a chalcone synthase gene from hairy root cultures of Scutellaria viscidula Bunge
Autores: | Lei,Wei; Tang,Shao-Hu; Luo,Ke-Ming; Sun,Min.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Chalcone synthase gene; Methyl jasmonate; Molecular cloning; Scutellaria viscidula Bunge.
Resumo: | A cDNA encoding chalcone synthase (CHS), the key enzyme in flavonoid biosynthesis, was isolated from hairy root cultures of Scutellaria viscidula Bunge by rapid amplification of cDNA ends (RACE). The full-length cDNA of S. viscidula CHS, designated as Svchs (GenBank accession no. EU386767), was 1649 bp with a 1170 bp open reading frame (ORF) that corresponded to a deduced protein of 390 amino acid residues, a calculated molecular mass of 42.56 kDa and a theoretical isoelectric point (pI) of 5.79. Multiple sequence alignments showed that SvCHS shared high homology with CHS from other plants. Functional analysis in silico indicated that SvCHS was a hydrophilic protein most likely associated with intermediate metabolism. The active sites of the malonyl-CoA binding motif, coumaroyl pocket and cyclization pocket in CHS of Medicago sativa were also found in SvCHS. Molecular modeling indicated that the secondary structure of SvCHS contained mainly α-helixes and random coils. Phylogenetic analysis showed that SvCHS was most closely related to CHS from Scutellaria baicalensis. In agreement with its function as an elicitor-responsive gene, the expression of Svchs was induced and coordinated by methyl jasmonate. To our knowledge, this is the first report to describe the isolation and expression of a gene from S. viscidula.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200015
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572010005000031
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.33 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess