Data/hora: 07/06/2024 07:56:10

Provedor de dados: | 74
País: | Brazil
Título: | Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosa
Autores: | Ceita,Geruza de Oliveira; Vilas-Boas,Laurival Antônio; Castilho,Marcelo Santos; Carazzolle,Marcelo Falsarella; Pirovani,Carlos Priminho; Selbach-Schnadelbach,Alessandra; Gramacho,Karina Peres; Ramos,Pablo Ivan Pereira; Barbosa,Luciana Veiga; Pereira,Gonçalo Amarante Guimarães; Góes-Neto,Aristóteles.
Data: | 2014-12-01
Ano: | 2014
Palavras-chave: | Basidiomycota; Fungus; Ergosterol; Theobroma cacao.
Resumo: | The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime &amp;amp; Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572014005000017
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.37 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess