Data/hora: 05/06/2024 15:23:09

Provedor de dados: | 74
País: | Brazil
Título: | Annexin II mRNA expression in bovine oocytes during follicular development
Autores: | Costa,Luis Fabiano Santos da; Machado,Márcia Silveira Netto; Oliveira,João Francisco Coelho de; Zamberlan,Gustavo; Gonçalves,Paulo Bayard Dias.
Data: | 2006-01-01
Ano: | 2006
Palavras-chave: | Bovine oocytes; Annexin II; Retinol.
Resumo: | We investigated the expression of calcium-dependent phospholipid binding protein annexin-II (Ann-II) messenger RNA (mRNA) during preantral follicle development and in oocytes from antral follicles of different diameters (&lt; 3 mm, 5 to 8 mm and &gt; 8 mm). The action of retinol on Ann-II mRNA expression in mature oocytes was also examined. Only oocytes from secondary preantral follicles expressed Ann-II mRNA and at the germinal vesicle stage expression by oocytes from follicles larger than 8 mm was significantly higher (p &lt; 0.05) compared with oocytes from follicles smaller than 3 mm or between 5 and 8 mm. Ann-II mRNA expression by metaphase II oocytes from follicles larger than 8 mm was significantly higher (p &lt; 0.05) than that from oocytes from follicles smaller than 3 mm, with oocytes from both these size-classes showing similar levels of Ann-II mRNA expression as oocytes recovered from 5-8 mm follicles. In the presence of retinol, Ann-II mRNA expression was higher than when retinol was absent (p &lt; 0.05). Our data indicate that Ann-II mRNA expression is highest in competent oocytes and that retinol increases Ann-II mRNA and may be involved in the regulation of oocyte competence by decreasing the translation and/or degradation of Ann-II mRNA.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200031
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572006000200031
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.29 n.2 2006
Fonte: | info:eu-repo/semantics/openAccess