Data/hora: 10/06/2024 01:14:36

Provedor de dados: | 74
País: | Brazil
Título: | Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
Autores: | Becker,Tânia C.A.; Chiuchetta,Simone J.R.; Baptista,Francielle; Castro-Prado,Marialba A.A. de.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Antineoplastic drug; Aspergillus nidulans; Ethidium bromide; Intercalating agent; Mitotic crossing over.
Resumo: | Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM). Results indicate that the intercalating agent is potentially capable of inducing mitotic crossing-over in diploid A. nidulans cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572003000300026
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.26 n.3 2003
Fonte: | info:eu-repo/semantics/openAccess