Data/hora: 06/06/2024 04:24:26

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Conversion of barley SNPs into PCR-based markers using dCAPS method	
Autores: 	Shahinnia,Fahimeh; Sayed-Tabatabaei,Badraldin Ebrahim.	
Data: 	2009-01-01	
Ano: 	2009	
Palavras-chave: 	Barley (Hordeum vulgare L.); Genome sequencing; Mismatched primer; Restriction enzyme; SNP genotyping.	
Resumo: 	Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572009005000047	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.32 n.3 2009	
Fonte: 	info:eu-repo/semantics/openAccess