Data/hora: 01/06/2024 14:30:25

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi	
Autores: 	Nora,Luísa Czamanski; Gonçales,Relber Aguiar; Martins-Santana,Leonardo; Ferreira,Beatriz Henriques; Rodrigues,Fernando; Silva-Rocha,Rafael.	
Data: 	2019-06-01	
Ano: 	2019	
Palavras-chave: 	Synthetic biology; Fungi; Agrobacterium tumefaciens; Transformation; Vectors.	
Resumo: 	Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300395	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/1678-4685-gmb-2018-0221	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.42 n.2 2019	
Fonte: 	info:eu-repo/semantics/openAccess