Data/hora: 05/06/2024 09:12:46

Provedor de dados: | 74
País: | Brazil
Título: | A two-step strategy for the complementation of M: tuberculosis mutants
Autores: | Movahedzadeh,Farahnaz; Frita,Rosangela; Gutka,Hiten J.
Data: | 2011-01-01
Ano: | 2011
Palavras-chave: | Tuberculosis; Mutagenesis; Constitutive expression; Complementation.
Resumo: | The sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively expressed dosR and have shown dosR expression is restored to wild-type level.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572011000200020
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.34 n.2 2011
Fonte: | info:eu-repo/semantics/openAccess