Data/hora: 07/06/2024 08:39:03

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts	
Autores: 	Zhang,Shumin; Xiong,Kai; Xie,Zhourui; Nan,Wenting; Liu,Honglin; Chen,Jie.	
Data: 	2012-01-01	
Ano: 	2012	
Palavras-chave: 	RNAi; ShRNA; Lentivirus; BLG; Goat.	
Resumo: 	β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-fetal fibroblast cells. Apart from high efficiency in the knockdown of BLG expression in these cells, lentivector-mediated RNAi manifested stable integration into the goat genome itself. Consequently, an in vitro model for goat BLG-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400020	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572012005000042	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.35 n.3 2012	
Fonte: 	info:eu-repo/semantics/openAccess