Data/hora: 16/06/2024 19:05:29

Provedor de dados: | 74
País: | Brazil
Título: | Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair
Autores: | Wits,Maria Inês; Tobin,Gabriela Cabanas; Silveira,Maiele Dornelles; Baja,Karine Gehlen; Braga,Luisa Maria Macedo; Sesterheim,Patricia; Camassola,Melissa; Nardi,Nance Beyer.
Data: | 2020-01-01
Ano: | 2020
Palavras-chave: | Mesenchymal stromal cells; Hyaluronic acid; Osteoarthritis; Dog.
Resumo: | Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100701
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2019-0275
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.43 n.1 2020
Fonte: | info:eu-repo/semantics/openAccess