Data/hora: 03/06/2024 06:21:44

Provedor de dados: | 74
País: | Brazil
Título: | Protein profile of rice (Oryza sativa) seeds
Autores: | Yang,Yanhua; Dai,Li; Xia,Hengchuan; Zhu,Keming; Liu,Haijun; Chen,Keping.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Mass spectrometry; Proteomic analysis; Rice seed; Two-dimensional electrophoresis.
Resumo: | Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572013000100012
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.36 n.1 2013
Fonte: | info:eu-repo/semantics/openAccess