Data/hora: 13/06/2024 07:41:32

Provedor de dados: | 74
País: | Brazil
Título: | A rapid alternative technique for obtaining silver-positive patterns in chromosomes
Autores: | Kavalco,Karine Frehner; Pazza,Rubens.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Silver staining; Ag-NOR; Microwaves; Chromosome banding.
Resumo: | Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results. Microwave irradiation proved to be quite efficient and reliable for this purpose, as it allowed to identify Ag-NORs equivalent to those obtained by the conventional procedure and also to reduce the concentration of the employed reagents, as well as the precipitation of debris on the preparation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200012
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572004000200012
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.27 n.2 2004
Fonte: | info:eu-repo/semantics/openAccess