Data/hora: 11/06/2024 09:17:25

Provedor de dados: 	74	
País: 	Brazil	
Título: 	The effect of magnesium on mitotic spindle formation in Schizosaccharomyces pombe	
Autores: 	Uz,Gulsen; Sarikaya,Aysegul Topal.	
Data: 	2016-09-01	
Ano: 	2016	
Palavras-chave: 	Magnesium; Microtubule; Mitosis; Mitotic spindle; Schizosaccharomyces pombe.	
Resumo: 	Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin construct to fluorescently label microtubules. Using the free Mg2+-specific fluorescent probe mag-fura-2, we confirmed that intracellular free Mg2+ levels were lower in GA2 than in the parental strain. Defects in interphase microtubule organization, a lower percentage of mitotic spindle formation and a reduced mitotic index were also observed in the GA2 strain. Although there was interphase microtubule polymerization, the lower level of mitotic spindle formation in the Mg2+-deficient strain suggested a greater requirement for Mg2+ in this phenomenon than previously thought.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/1678-4685-GMB-2015-0239	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.39 n.3 2016	
Fonte: 	info:eu-repo/semantics/openAccess