Data/hora: 10/06/2024 10:28:36

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Array-CGH testing in spontaneous abortions with normal karyotypes	
Autores: 	Borovik,Cleide L.; Perez,Ana Beatriz A.; Silva,Luciana R.J. da; Krepischi-Santos,Ana Cristina V.; Costa,Silvia S.; Rosenberg,Carla.	
Data: 	2008-01-01	
Ano: 	2008	
Palavras-chave: 	Spontaneous abortion; Chromosomal aberrations; Array-CGH.	
Resumo: 	In about 50% of first trimester spontaneous abortion the cause remains undetermined after standard cytogenetic investigation. We evaluated the usefulness of array-CGH in diagnosing chromosome abnormalities in products of conception from first trimester spontaneous abortions. Cell culture was carried out in short- and long-term cultures of 54 specimens and cytogenetic analysis was successful in 49 of them. Cytogenetic abnormalities (numerical and structural) were detected in 22 (44.89%) specimens. Subsequent, array-CGH based on large insert clones spaced at ~1 Mb intervals over the whole genome was used in 17 cases with normal G-banding karyotype. This revealed chromosome aneuplodies in three additional cases, giving a final total of 51% cases in which an abnormal karyotype was detected. In keeping with other recently published works, this study shows that array-CGH detects abnormalities in a further ~10% of spontaneous abortion specimens considered to be normal using standard cytogenetic methods. As such, array-CGH technique may present a suitable complementary test to cytogenetic analysis in cases with a normal karyotype.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300004	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572008000300004	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.31 n.2 2008	
Fonte: 	info:eu-repo/semantics/openAccess