Data/hora: 01/06/2024 00:51:46

Provedor de dados: | 74
País: | Brazil
Título: | Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach
Autores: | Rossi,Ciro César; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de; Bazzolli,Denise Mara Soares.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Actinobacillus pleuropneumoniae; OmlA gene; Phylogenetic reconstruction; Porcine pleuropneumonia.
Resumo: | The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A conserved α-helix common to all the serotypes was most likely involved in connecting the protein to the outer membrane and acting as a signal peptide. A previously unknown gene duplication was also identified and could contribute to the genetic variability that makes it difficult to serotype some isolates. Our data support the importance of the omlA gene in the biology of A. pleuropneumoniae and provide a new area of research into the OmlA protein.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200015
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572013005000012
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.36 n.2 2013
Fonte: | info:eu-repo/semantics/openAccess