Data/hora: 01/06/2024 02:17:39

Provedor de dados: | 74
País: | Brazil
Título: | Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
Autores: | Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Valdez Ramírez,Carlos; Gómez Gallardo,David; León Sánchez,Rafael; Canales Aguirre,Alejandro; Feria Velasco,Alfredo.
Data: | 2014-01-01
Ano: | 2014
Palavras-chave: | Comet assay; DNA damage; Genotoxicity; Glyphosate.
Resumo: | There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 µM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p &lt; 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p &lt; 0.001) genotoxicity was observed at &amp;gt; 7 µM, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at &amp;gt; 0.7 µM. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7-7 µM.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100016
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572014000100016
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.37 n.1 2014
Fonte: | info:eu-repo/semantics/openAccess