Data/hora: 11/06/2024 06:50:45

Provedor de dados: | 74
País: | Brazil
Título: | Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum
Autores: | Monteiro,Rita; Carvalho,Cláudia; Collares-Pereira,Maria João.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Ag/CMA3-banding; Flow cytometry; NORs; Chromosome polymorphism; Spontaneous triploidy.
Resumo: | This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I. lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I. lusitanicum specimen) and a corresponding haploid chromosome formula of 7M:15SM:3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I. lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both between and within species: the genome of I. almacai was smaller than that of I. lusitanicum (mean values 2.61 and 2.93 pg, respectively), which presented a clear interpopulational variability (mean values ranging from 2.72 to 3.00 pg). These data allowed the distinction of both taxa and confirmed the existence of two well differentiated groups within I. lusitanicum: one that includes the populations from the right bank of the Tejo and Samarra drainages, and another that reunites the southern populations. The peculiar differences between the two species, presently listed as "Critically Endangered", reinforced the importance of this study for future conservation plans.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200011
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009000200011
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess