Data/hora: 01/06/2024 11:16:49

Provedor de dados: | 74
País: | Brazil
Título: | Comparison of base substitutions in response to nitrogen ion implantation and 60Co-gamma ray irradiation in Escherichia coli
Autores: | Chuan-Xiao,Xie; An,Xu; Li-Jun,Wu; Jian-Min,Yao; Jian-Bo,Yang; Zeng-Liang,Yu.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Base substitution; Low-energy nitrogen ion beam implantation; 60Co-gamma rays; Escherichia coli.
Resumo: | To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif r) mutant screening and sequencing of the defined region of the rpoB gene in E. coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or 60Co-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transitions were significantly increased from 32 to 46, AT &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transitions, AT &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;GC transitions, AT &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by 60Co-gamma rays were preferentially GC &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;AT and AT &lt;FONT FACE=Symbol&gt;®&lt;/font&gt;GC transitions, which totaled 84.31% (43/51).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200025
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572004000200025
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.27 n.2 2004
Fonte: | info:eu-repo/semantics/openAccess