Data/hora: 06/06/2024 15:42:57

Provedor de dados: | 74
País: | Brazil
Título: | Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform
Autores: | Xiaojun,Fan; Chun,Yang; Jianhong,Liu; Chang,Zhang; Yao,Li.
Data: | 2017-03-01
Ano: | 2017
Palavras-chave: | Asian gypsy moth; Chitin metabolism; Transcriptome; Unigenes.
Resumo: | Abstract The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI non-redundant protein database and 9,613 were annotated in the Swiss-Prot database. We mapped 4,329 unigenes onto 317 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. Chitin metabolism unigenes were found in the transcriptome and the data indicated that a variety of enzymes was involved in chitin catabolic and biosynthetic pathways.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100160
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2015-0257
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.40 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess