Data/hora: 01/06/2024 05:48:30

Provedor de dados: | 74
País: | Brazil
Título: | Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus)
Autores: | Pinheiro,Janeth Silva; Wolff,José Luiz Caldas; Araújo,Ronaldo de Carvalho; Hilsdorf,Alexandre Wagner Silva.
Data: | 2008-01-01
Ano: | 2008
Palavras-chave: | Piaractus mesopotamicus; Growth hormone; CDNA cloning; Sequencing.
Resumo: | RT-PCR was used for amplifying Piaractus mesopotamicus growth hormone (GH) cDNA obtained from mRNA extracted from pituitary cells. The amplified fragment was cloned and the complete cDNA sequence was determined. The cloned cDNA encompassed a sequence of 543 nucleotides that encoded a polypeptide of 178 amino acids corresponding to mature P. mesopotamicus GH. Comparison with other GH sequences showed a gap of 10 amino acids localized in the N terminus of the putative polypeptide of P. mesopotamicus. This same gap was also observed in other members of the family. Neighbor-joining tree analysis with GH sequences from fishes belonging to different taxonomic groups placed the P. mesopotamicus GH within the Otophysi group. To our knowledge, this is the first GH sequence of a Neotropical characiform fish deposited in GenBank.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200037
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008000200037
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.1 suppl.0 2008
Fonte: | info:eu-repo/semantics/openAccess