Data/hora: 08/06/2024 18:52:36

Provedor de dados: | 74
País: | Brazil
Título: | Satellite DNA sites in four species of the genus Astyanax (Teleostei, Characiformes)
Autores: | Kavalco,Karine Frehner; Pazza,Rubens; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Astyanax; Fluorescence in situ hybridization (FISH); Heterochromatin; Karyotypic diversity; Satellite DNA.
Resumo: | Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly restricted to the terminal and pericentromeric regions of a few chromosomes. No GC-positive regions, neither any correspondence between the scarce heterochromatin of these species and the As-51 satellite DNA was observed. AT-positive blocks were not detected in any of the species studied. Based on these and other available data, the hypothesis that Astyanax represents a polyphyletic group is discussed.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000400005
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.3 2007
Fonte: | info:eu-repo/semantics/openAccess