Data/hora: 05/06/2024 18:25:28

Provedor de dados: | 74
País: | Brazil
Título: | Hematoxylin: a simple, multiple-use dye for chromosome analysis
Autores: | Guerra,Marcelo.
Data: | 1999-03-01
Ano: | 1999
Resumo: | A staining mixture of hematoxylin-iron alum combined with a strong hydrochloric hydrolysis was successfully applied for chromosome observation of several kinds of plants and some animals. Slightly different procedures were developed for different materials and objectives. For plant cells, the most important technical aspect was the use of 5 N HCl hydrolysis, which resulted in a very transparent cytoplasm, combined with an intense, specific hematoxylin stain. This technique is recommended for cytogenetical analysis in general, and it is especially indicated for practical classes, due to its simplicity and high reproducibility of results. Moreover, the deep contrast observed makes this technique very useful for sequential staining of cells previously analyzed with other stains, as well as for materials with fixation problems.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100016
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47571999000100016
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.22 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess