Data/hora: 07/06/2024 03:58:35

Provedor de dados: | 74
País: | Brazil
Título: | Mitochondrial variability in the D-loop of four equine breeds shown by PCR-SSCP analysis
Autores: | Mirol,Patricia M.; Peral García,P.; Dulout,F.N.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | MTDNA; Equine; PCR.
Resumo: | A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbred, TB; Argentinian Creole, ARC; and Peruvian Paso from Argentina, PPA). Each breed showed four to eight SSCP variants, many of which were shared between two or three of the studied breeds. Arabian horses were the most variable (eigth variants), with three variants unique to the breed. PPA and ARC showed two and one characteristic SSCP variants, respectively, while TB shared all its variants with at least one of the other breeds. An analysis based on the presence/absence of the variants revealed a closer relationship between PPA and TB, which was not completely unexpected considering the mixed ancestry of the PPA mares. The results also confirm the efficiency of SSCP to detect variability in horse mitochondrial DNA.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100006
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572002000100006
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.25 n.1 2002
Fonte: | info:eu-repo/semantics/openAccess