Data/hora: 07/06/2024 02:35:38

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean	
Autores: 	Pereira,Wendell Jacinto; Melo,Arthur Tavares de Oliveira; Coelho,Alexandre Siqueira Guedes; Rodrigues,Fabiana Aparecida; Mamidi,Sujan; Alencar,Sérgio Amorim de; Lanna,Anna Cristina; Valdisser,Paula Arielle Mendes Ribeiro; Brondani,Claudio; Nascimento-Júnior,Ivanildo Ramalho do; Borba,Tereza Cristina de Oliveira; Vianello,Rosana Pereira.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	Phaseolus vulgaris; Water deficit; Functional genomics; Single nucleotide polymorphism (SNP).	
Resumo: 	Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/1678-4685-gmb-2018-0259	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.43 n.1 2020	
Fonte: 	info:eu-repo/semantics/openAccess