Data/hora: 09/06/2024 02:59:57

Provedor de dados: | 74
País: | Brazil
Título: | Genetic diversity analysis of common beans based on molecular markers
Autores: | Gill-Langarica,Homar R.; Muruaga-Martínez,José S.; Vargas-Vázquez,M.L. Patricia; Rosales-Serna,Rigoberto; Mayek-Pérez,Netzahualcoyotl.
Data: | 2011-01-01
Ano: | 2011
Palavras-chave: | Phaseolus vulgaris L.; Amplified fragment length polymorphisms (AFLP); Germplasm bank; Genetic relationships; Simple sequence repeats (SSR).
Resumo: | A core collection of the common bean (Phaseolus vulgaris L.), representing genetic diversity in the entire Mexican holding, is kept at the INIFAP (Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Mexico) Germplasm Bank. After evaluation, the genetic structure of this collection (200 accessions) was compared with that of landraces from the states of Oaxaca, Chiapas and Veracruz (10 genotypes from each), as well as a further 10 cultivars, by means of four amplified fragment length polymorphisms (AFLP) +3/+3 primer combinations and seven simple sequence repeats (SSR) loci, in order to define genetic diversity, variability and mutual relationships. Data underwent cluster (UPGMA) and molecular variance (AMOVA) analyses. AFLP analysis produced 530 bands (88.5% polymorphic) while SSR primers amplified 174 alleles, all polymorphic (8.2 alleles per locus). AFLP indicated that the highest genetic diversity was to be found in ten commercial-seed classes from two major groups of accessions from Central Mexico and Chiapas, which seems to be an important center of diversity in the south. A third group included genotypes from Nueva Granada, Mesoamerica, Jalisco and Durango races. Here, SSR analysis indicated a reduced number of shared haplotypes among accessions, whereas the highest genetic components of AMOVA variation were found within accessions. Genetic diversity observed in the common-bean core collection represents an important sample of the total Phaseolus genetic variability at the main Germplasm Bank of INIFAP. Molecular marker strategies could contribute to a better understanding of the genetic structure of the core collection as well as to its improvement and validation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400012
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572011005000056
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.34 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess