Data/hora: 11/06/2024 11:58:52

Provedor de dados: | 74
País: | Brazil
Título: | Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds
Autores: | Mazzi,Carmen Maria; Ferro,Jesus Aparecido; Ferro,Maria Inês Tiraboschi; Savino,Vicente José Maria; Coelho,Antonio Augusto Domingos; Macari,Marcos.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Broiler chickens; Hsp70 gene; Naked neck (Na) gene; Polymorphism; SSCP.
Resumo: | The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding region and the sequencing of the PCR products confirmed and identified two polymorphic sites in this region: a transition A ® G in position +258 and a transversion C ® G in position +276. Both mutations were considered to be silent, since they did not modify the aminoacid sequence of the protein Hsp70. The promoter region of the hsp70 gene was identical in all studied birds, indicating that the regulation pattern of this gene must be the same in all birds at the promoter region. Three different alleles (hsp70-1, hsp70-2 and hsp70-3) were identified for the hsp70 gene from the observed mutations. The allele hsp70-3 was detected in only two breeds, Hubbard-Pettersen and PP1, but at a low frequency (0,016 and 0,006, respectively).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300010
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572003000300010
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.26 n.3 2003
Fonte: | info:eu-repo/semantics/openAccess