Data/hora: 12/06/2024 14:08:48

Provedor de dados: | 74
País: | Brazil
Título: | Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization
Autores: | Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Citrus; Disease resistance; Citrus tristeza virus; Biotic stress.
Resumo: | Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs (Tentative Consensi sequences) were obtained using both cDNA libraries in a single clusterization procedure. By the in silico hybridization approach, 289 TCs were identified as differentially expressed in the two libraries. A total of 121 TCs were found to be overexpressed in plants infected with CTV and were grouped in 12 primary functional categories. The majority of them were associated with metabolism and defense response. Some others were related to lignin, ethylene biosynthesis and PR proteins. In general, the differentially expressed transcripts seem to be somehow involved in secondary plant response to CTV infection.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000500025
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.3 suppl.0 2007
Fonte: | info:eu-repo/semantics/openAccess