Data/hora: 11/06/2024 06:49:41

Provedor de dados: | 74
País: | Brazil
Título: | An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders
Autores: | Sastre,Darío A.; Argaraña,Carlos E.; Heller,Viviana B.; Gallo,Mónica; Fernández,Enrique N.; Rodríguez,Cecilia M.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Multiplex-PCR; Ph chromosome; BCR-ABL; Chronic myeloid leukemia; PCR.
Resumo: | In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for e1a2, while in the BCR-ABL(+)AML patients a b3a2 rearrangement was observed. In conclusion, multiplex-PCR allows rapid, specific and simultaneous detection of different types of BCR-ABL transcripts in CML and ABL-BCR(+)AL. A full correlation was observed when multiplex-PCR was compared with nested PCR.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400003
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000400003
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.3 2007
Fonte: | info:eu-repo/semantics/openAccess