Data/hora: 10/06/2024 15:56:18

Provedor de dados: | 75
País: | Brazil
Título: | Genetic improvement of horticultural crops mediated by CRISPR/Cas: a new horizon of possibilities
Autores: | Koltun,Alessandra; Erpen-Dalla Corte,Lígia; Mertz-Henning,Liliane Marcia; Gonçalves,Leandro SA.
Data: | 2018-09-01
Ano: | 2018
Palavras-chave: | Genome editing; Plant breeding; Biotechnology; Induced mutations; Non-transgenic.
Resumo: | ABSTRACT The burden of the current global challenge involving food security lies in the need to improve crop production. In this regard, biotechnology stands out as an essential tool to generate plants able to cope with pests, diseases, and harsh climatic conditions, and more efficient in the use of natural resources. An advanced approach to create genetic variability in a precise and targeted way, the genome-editing technique CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins), has drawn the attention of breeders. The genome editing CRISPR/Cas system relies on a guiding RNA that directs a nuclease to generate a double-strand break (DSB) at a target DNA, activating the cell repair systems and eventually leading to deletions or insertions of nucleotides. Therefore, CRISPR/Cas is a toolbox to achieve many goals, from basic science investigations to the development of crops with improved agronomic traits, with potential to bring innovative solutions to food production. The CRISPR/Cas system has been applied in a large number of plants, including some horticultural species. In this review, we present details of the CRISPR/Cas natural and artificial systems, its possibilities as a biotechnological tool, advantages over other breeding techniques, regulatory issues, and its applicability in horticultural crops, as well as future challenges.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300290
Editor: | Associação Brasileira de Horticultura
Relação: | 10.1590/s0102-053620180302
Formato: | text/html
Fonte: | Horticultura Brasileira  v.36 n.3 2018
Fonte: | info:eu-repo/semantics/openAccess