Data/hora: 16/06/2024 01:43:02

Provedor de dados: | 75
País: | Brazil
Título: | In vitro culture of jambu with different growth regulators
Autores: | Almeida,Susan P; Souza,Joanne MM; Amorim,Andredy MT; Gusmão,Sérgio AL de; Souza,Rodrigo ORM; Santos,Alberdan S.
Data: | 2020-04-01
Ano: | 2020
Palavras-chave: | Acmella oleracea; Micropropagation; Callus induction; BAP; 2.4-D; Nodal segments.
Resumo: | ABSTRACT The aim of this study was to establish the best concentrations of growth regulators for in vitro cultivation of jambu for a subsequent elaboration of an efficient micropropagation protocol. After sterilized, the seeds were inoculated on different media (MS, ½MS and water-agar) for in vitro germination. Nodal segments of in vitro germinated jambu seedlings were used as explants in the micropropagation with different concentrations of 6-benzylaminopurine (BAP) (0.0; 0.125; 0.25; 0.50 and 0.75 mg L-1) and callus induction with 2.4- dichlorophenoxyacetic acid (2.4-D) (0.0; 0.25; 0.50; 0.75 and 1.0 mg L-1) on Murashige &amp; Skoog’s (MS) medium. The highest germination rates were obtained on MS medium with better seedling development and greater height (3.7cm). In micropropagation, the best treatment was obtained on 0.125 BAP (T2), with an average of 2.2 sprouts/explant, average height of 2.4 cm and vigorous sprouts. In callus induction, all treatments with 2.4-D had developed friable calluses in 30 days and using doses of 0.25 and 0.50 mg L-1 provided greater fresh matter. The induction of friable callus and the root production occur without supplementation of exogenous growth regulator.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200134
Editor: | Associação Brasileira de Horticultura
Relação: | 10.1590/s0102-053620200204
Formato: | text/html
Fonte: | Horticultura Brasileira  v.38 n.2 2020
Fonte: | info:eu-repo/semantics/openAccess