Data/hora: 10/06/2024 16:04:47

Provedor de dados: | 75
País: | Brazil
Título: | Extreme resistance to two Brazilian strains of Potato virus Y (PVY) in transgenic potato, cv. Achat, expressing the PVYº coat protein
Autores: | Romano,Eduardo; Ferreira,Adriana T.; Dusi,André N.; Proite,Karina; Buso,Jose A.; Ávila,Antonio C.; Nishijima,Marta L.; Nascimento,Adriana S.; Bravo-Almonacid,Fernando; Mentaberry,Alejandro; Monte,Damares; Campos,Magnólia A.; Melo,Paulo Eduardo; Cattony,Monica K.; Torres,Antonio C.
Data: | 2001-07-01
Ano: | 2001
Palavras-chave: | Solanum tuberosum; GMO.
Resumo: | The coat protein (CP) gene of the potato virus Y strain "o" (PVY O) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during several in vitro multiplications over a period of three years, with no modification in the Southern pattern was observed. The stability of the transgenes, the absence of primary infections and the relatively broad spectrum of resistance suggest that the extremely resistant line obtained in this work can be useful for agricultural purposes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362001000200004
Editor: | Associação Brasileira de Horticultura
Relação: | 10.1590/S0102-05362001000200004
Formato: | text/html
Fonte: | Horticultura Brasileira  v.19 n.2 2001
Fonte: | info:eu-repo/semantics/openAccess