Data/hora: 10/06/2024 03:51:41

Provedor de dados: | 77
País: | Chile
Título: | Histological Changes of the Ovary of Adult Mice Exposed Prenatally to Flunitrazepam
Autores: | Aguirre -Samudio,Ana Julia; de la Fuente-Juárez,Graciela; Márquez-Orozco,Amalia; Márquez-Orozco,María Cristina.
Data: | 2006-01-01
Ano: | 2006
Palavras-chave: | Benzodiazepine; Flunitrazepam; Atretic follicles; Corpus luteum; Prenatal exposure.
Resumo: | Flunitrazepam (FNZ) is a minor tranquillizer involving allosteric modulation of the GABA receptor complex. It is a ligand of the peripheral benzodiazepine receptor (PBR) that participates in cholesterol transport in steroidogenic organs. The purpose was to investigate whether a single oral dose of FNZ of 2.5 mg/kg of body weight (bw), administered on day 6 of gestation, alters the structure of the adult ovary of mouse offsprings at 2 months of age. The mouse offsprings of the in utero FNZ-treated group and those of the control group (C) were killed. Ovaries were obtained in early estrous, fixed in Zenker solution, and processed by routine histological techniques. Serial sections were observed under light microscopy to determine the characteristics and quantity of follicles in different stages of development and of the corpus luteum. The ovarian tissues from the FNZ group depicted a great staining affinity, enlarged nuclei with abundant heterochromatin clumps. The quantity of primordial, primary and secondary normal follicles in the FNZ group decreased significantly (p&lt; 0.01). The number of primary atretic follicles increased (p&lt;0.01) and the secondary ones remained constant as in group C. Histological changes and statistical data suggest that FNZ produces long-lasting epigenetic or genotoxic effects in follicular and corpus luteum cells of the ovary of prenatally FNZ-treated mice
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500022
Editor: | Sociedad Chilena de Anatomía
Formato: | text/html
Fonte: | International Journal of Morphology  v.24 n.4 2006