Data/hora: 09/06/2024 11:45:52

Provedor de dados: 	79	
País: 	Brazil	
Título: 	In vitro assay of biological and chemical toxins using antibodies against lethal toxin neutralizing factor	
Autores: 	LIPPS,B. V.	
Data: 	2002-01-01	
Ano: 	2002	
Palavras-chave: 	Antibodies; In vitro assay; Lethal toxin neutralizing factor; Toxins; Venoms.	
Resumo: 	Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently, mouse bioassay is in use for toxin detection and assay. Binding affinity of IgG from anti-LT-10 showed a linear relationship with mouse bioassay by ELISA detection limit to some toxins only. This may be due to the fact that anti-LTNF and anti-LT-10 detected the toxins that were not lethal to mouse. Thus, anti-LTNF and anti-LT-10 can be useful in assaying toxins as an alternative to mouse bioassay.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200003	
Editor: 	Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP	
Relação: 	10.1590/S0104-79302002000200003	
Formato: 	text/html	
Fonte: 	Journal of Venomous Animals and Toxins  v.8 n.2 2002	
Fonte: 	info:eu-repo/semantics/openAccess