Data/hora: 09/06/2024 16:41:12

Provedor de dados: | 80
País: | Brazil
Título: | Biotin-avidin sandwich elisa with specific human isotypes IgG1 and IgG4 for Culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil
Autores: | Marassá,AM; Paula,MB; Gomes,AC; Consales,CA.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Culicidae; ELISA; Feeding habits; Isotypes; Yellow fever.
Resumo: | With a view toward investigating the feeding behavior of Culicidae mosquitoes from an area of epizootic yellow fever transmission in the municipalities of Garruchos and Santo Antônio das Missões, Rio Grande do Sul State, Brazil, specimens were collected by aspiration from September 2005 to April 2007. The engorged females were submitted to blood meal identification by enzyme-linked immunosorbent assay (ELISA). A total of 142 blood-engorged samples were examined for human or monkey blood through species-specific IgG. Additional tests for specificity utilizing isotypes IgG1 and IgG4 of human monoclonal antibodies showed that only anti-human IgG1 was effective in recognizing blood meals of human origin. The results indicated a significant difference (p = 0.027) in detection patterns in samples of Haemagogus leucocelaenus recorded from human blood meals at Santo Antônio das Missões, which suggests some degree of exposure, since it was an area where epizootic outbreaks have been reported.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400008
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992009000400008
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.15 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess