Data/hora: 15/06/2024 03:08:18

Provedor de dados: | 80
País: | Brazil
Título: | A novel lipocalin homologue from the venom gland of Deinagkistrodon acutus similar to mammalian lipocalins
Autores: | Wei,CB; Chen,J.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | Deinagkistrodon acutus; CDNA library; Lipocalin; Phylogeny; Gene expression pattern analysis.
Resumo: | Lipocalins are involved in a variety of functions including retinol transport, cryptic coloration, olfaction, pheromone transport, prostaglandin synthesis, regulation of the immune response and cell homeostatic mediation. A full-length cDNA clone (named d-lipo), isolated from the venom gland cDNA library of Deinagkistrodon acutus, contained an insert of 664 bp including an open reading frame that encodes a lipocalin homologue of 177 amino acids. Comparison of d-lipo and other related proteins revealed an overall amino acid identity of less than 21.5%. Primary structures of d-lipo carried three structurally conserved regions (SCR) showing homologies to those of lipocalins. The first conserved Cys residue - the essential amino acid residue for the catalytic activity and unique to lipocalin-type prostaglandin D synthase (L-PGDS) in the lipocalin protein family - was identified in d-lipo at amino acid position 58. Phylogenetic tree analysis showed that d-lipo was in-between the large L-PGDS cluster and the small von Ebner's-gland proteins (VEGP) cluster. Moreover, d-lipo gene presented a high-level expression in the venom gland and a low-level expression in the brain and its expression was significantly increased under pathological conditions, suggesting a possible relationship between d-lipo mRNA expression and the venom gland inflammatory disease. This is also the first report of a lipocalin homologous gene identified in the venom gland of a snake.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100003
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992012000100003
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.18 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess