Data/hora: 01/06/2024 08:32:48

Provedor de dados: | 80
País: | Brazil
Título: | Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua
Autores: | Sato,Ana Claudia; Bosch,Rosemary Viola; Will,Sonia Elisabete Alves; Alvarez-Flores,Miryam Paola; Goldfeder,Mauricio Barbugiani; Pasqualoto,Kerly Fernanda Mesquita; Silva,Bárbara Athayde Vaz Galvão da; Andrade,Sonia Aparecida de; Chudzinski-Tavassi,Ana Marisa.
Data: | 2016-01-01
Ano: | 2016
Palavras-chave: | Hemolin; RLosac; Wound healing; Collagen.
Resumo: | Abstract  Background  Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin’s roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar Lonomia obliqua, presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac’s in vivo effects using a skin wound healing model in rats.  Methods  Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the following tissue regeneration parameters were evaluated: wound closure and collagen content. Furthermore, tissue sections were subjected to histological and immunohistochemical analyses.  Results  The rLosac treatment has demonstrated its capacity to improve wound healing, as reflected in findings of a larger number of activated fibroblasts, proliferation of epithelial cells, increase of collagen type 1, and decrease of inflammatory infiltrate.  Conclusion  The findings have indicated the rLosac protein as a very promising molecule for the development of new wound-healing formulations.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100324
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1186/s40409-016-0093-4
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.22 2016
Fonte: | info:eu-repo/semantics/openAccess