Data/hora: 06/06/2024 05:35:12

Provedor de dados: | 80
País: | Brazil
Título: | Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study
Autores: | Baldo,C; Ferreira,MJ; Lopes,DS; Izidoro,LFM; Gomes,AO; Ferro,EAV; Hamaguchi,A; Homsi-Brandeburgo,MI; Rodrigues,VM.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Skeletal muscle; Neuwiedase; Snake venom metalloproteinases; Bothrops neuwiedi.
Resumo: | The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992010000300013
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.16 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess