Data/hora: 15/06/2024 21:27:28

Provedor de dados: | 80
País: | Brazil
Título: | Detection of bovine Clostridium perfringens by polymerase chain reaction
Autores: | Piatti,R. M.; Ikuno,A. A.; Baldassi,L.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Clostridium perfringens; PCR; Alpha toxin; Polymerase chain reaction.
Resumo: | A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992004000200005
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.10 n.2 2004
Fonte: | info:eu-repo/semantics/openAccess